O. Torrissen and O.R. Braekkan
Proc. World Symp. On Finfish Nutrition and Fishfeed Technology, Hamburg 20-23 June, 1978. Vol. II. Berlin 1979.
Astaxanthin was isolated from lipid extracts of raw calanus (Calanus finmarchicus) as diester, monoester and free forms by column chromatography. The different forms were dissolved in a marine oil and mixed in moist pellets fed to rainbow trout (Salmo gairdneri). Raw calanus was fed to one group for comparison.
Samples were taken at 19 and 53 days and analyzed for muscle pigmentation.
The results showed the best pigment retention in the group fed free astaxanthin, and the lowest values were obtained in the groups with astaxanthin diester.